A cross sectional study was conducted from October 2014 to April 2015 to determine prevalence of wound and associated risk factors in working donkeys in and around Merawi district, Amhara region, Ethiopia. A total of 384 randomly selected donkeys were physically examined for any external body injury/wound. Simultaneously, donkey owners were interviewed with a pre-tested semi-structured questionnaire to extrapolate information regarding possible risk factors associated with wound occurrence. The overall prevalence of wound was 38.3%. The occurrence of wound varied significantly among donkeys with different age categories (χ2 = 43.027, p < 0.05) and with different body score conditions (47.5%, χ2 = 53.8, OR = 34.89, CI = 8.4-144.6). However the occurrence of wounds didn’t vary significantly among sex of the animals, type of pack saddle used and type of load carried (p > 0.05). In terms of working nature, donkeys working for more than 10kms per single trip showed

higher prevalence of wound (50.0%, χ2 = 9.39, p < 0.05) than those usually working for nearer distances (<10kms). There was a signifi- cant difference in the severity of wound among affected donkeys carrying different loads (χ2 = 26.71, p < 0.05).Questionnaire survey among the 384 respondents showed only 51% of the respondents usually seek for veterinary help for wound management. Generally, the study has clearly indicated wound as a prevailing welfare problem of working donkeys in and around Merawi district. Hence, implementing a comprehensive donkey health and welfare improvement program should be a priority for concerned stakeholder.